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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Latitudinal effect on mosquitoes' microbiota
 LEIBETSEDER, Melissa
The aim of this thesis was to find environmental influences on the microbiota of Cx. pipiens mosquito, while investigating the 16S ribosomal RNA composition of the samples, collected over different latitudes in Europe.
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Morfocytometric investigation of haemocytes in comparative hematology
 ŠUBJAK, David
Standard haematological procedures in preclinical toxicity studies are mostly carried out on mammals, more recent haematotoxicological models represent invertebrates because they can be used in the screening of myelotoxicity. In the present study, we examined haemocytes of Culex pipiens as a new potential alternative haematological in vivo model. Preparations for microscopic analysis were made from the hemolymph stained by Pappenheim's method for overstriking morphology and by toluidin blue and AgNOR for overstriking nucleoles. Microscopic photos were processed by image analysis and size of cells, nuclei and nucleoli were evaluated. Six types of haemocytes were found: granulocytes, prohemocytes, spherulocytes, plasmatocytes, adipohemocytes and oenocytes. Thanks to image analysis we made a morfometric profile of each type of haemocytes and set the nucleolar coefficient. We described significant differences among prohemocytes, plasmocytes and oenocytes size. On the grounds of nucleoles size we divided haemocytes to next two significant groups. Image analysis seems to be very helpful for distinction among various haemocyte lines but we cant use it for generally separation without personal control thanks to presence of statistically significant findings for only some comparisons cell types.
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